Biolegend, 113006, APC anti-mouse CD120a (TNF R Type I/p55) Antibody, 100μg

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody. Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Pinckard JK, et al. 1997. J. Biol Chem. 272:10784. (IP)Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)Bell JH, et al. 2007. J. Leukocyte Biol. 82:173. (FC)Kuroishi T, et al. 2008. J. Leukocyte Biol. 83:912.de Oliveria DC, et al. 2014. Cytokine. 69:218. PubMedKumari S, et al. 2014. Elife. 3:3422. PubMed
Product Citations: Adelaja A, et al. 2021. Immunity. 54(5):916-930.e7. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed Xiao Z, et al. 2022. Cell Death Dis. 13:885. PubMed Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Kumari S, et al. 2014. Elife. 3:3422. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Oliveira D, et al. 2014. Cytokine. 69:218. PubMed Desu HL, et al. 2021. Cells. 10:. PubMed
RRID: AB_2208780 (BioLegend Cat. No. 113005) AB_2208779 (BioLegend Cat. No. 113006)
Structure: TNFR superfamily, 55 kD
Distribution: Variety of cell types at low levels
Function: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor: TNF-α, LT-α (TNF-β)
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Aggarwal BB, et al. 1985. Nature 318 665. 2. Chan FKM, et al. 2000. Science 288:2351. 3. Loetscher H, et al. 1990. Cell 61:351. 4. Rothe J, et al. 1993. Nature 364:798.
Gene ID: 21937
UniProt: View information about CD120a on UniProt.org
Clone: 55R-286
Regulatory Status: RUO
Other Names: TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype: Armenian Hamster IgG