Biolegend, 114007, Brilliant Violet 421™ anti-mouse CCRL2 Antibody, 50μg

CCRL2 (C-C chemokine receptor-like 2) is also known as Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR/Lccr). CCRL2 is a chemokine receptor-like, 7-transmembrane protein that can bind CCL19 or chemerin/RARRES2. Mouse CCRL2 is expressed by several leukocytes, including dendritic cells, macrophages, neutrophils, and microglia and is rapidly upregulated following stimulation with proinflammatory stimuli, such as LPS and TNF-α. CCRL2 plays various roles in regulating chemokine activities, e.g., by binding and internalizing excess local levels of the CCL19/MIP-3β chemokine. Alternatively, endothelial cell-expressed CCRL2 can bind & present the chemerin/RARRES2 chemoattractant to lymphoid cells that express the functional signaling receptor, CMKLR1, and promote their adhesion.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCRL2-Transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S21013E completely blocks the reference clone BZ2E3.
RRID: AB_3106061 (BioLegend Cat. No. 114007)
Structure: C-C chemokine receptor-like 2 (CCRL2) is a non-signaling seventransmembrane domain (7-TMD) receptor related to the atypical chemokine receptor (ACKR) family.
Distribution: Expressed in many leukocytes, including dendritic cells, macrophages, neutrophils, mast cells, microglia & astrocytes
Function: CC-chemokine receptor-like 2 (CCRL2) binds leukocyte chemoattractant chemerin and can regulate local levels of the attractant, but does not itself support cell migration.
Interaction: Macrophages, Dendritic Cells, Mast Cells
Ligand/Receptor: CCL19 and chemerin/RARRES2 bind to CCRL2
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Monnier J, et al. 2012. J Immunol. 2012; 189:956-67. Shimada T, et al. 1998. FEBS Lett. 1998; 425:490-4 Yoshimura T, et al. 2011. Exp Cell Res. 317:674-84. Zabel BA, et al. 2008. J Exp Med. 205:2207-20
Gene ID: 54199
UniProt: View information about CCRL2 on UniProt.org
Clone: S21013E
Regulatory Status: RUO
Other Names: C-C chemokine receptor-like 2, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR), Cmkbr1l2, Ackr5
Isotype: Rat IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.