Biolegend, 114405, FITC anti-mouse I-A, 50μg

The 25-9-17 antibody reacts with the beta chain of the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2 d, p, q haplotypes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.
Application References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (IP) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. Ozato K, et al. 1981. J. Immunol. 126:317. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC) Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC) Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.
Product Citations: Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
RRID: AB_313581 (BioLegend Cat. No. 114406)
Structure: ß chain of I-Ab MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961
UniProt: View information about I-Ab on UniProt.org
Clone: 25-9-17
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (C3H) IgG2a, κ