Biolegend, 114502, Purified anti-mouse H-2L, 500μg

The 28-14-8 antibody reacts with the H-2D b MHC class I alloantigen expressed on nucleated cells from mice of the H-2D b haplotype. H-2D b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2L d haplotype. Clone 28-14-8 recognizes the α3 domain of H-2D b .
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114510). Does not react with other haplotypes (e.g., k, f, p, r, s).
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 125:2473. Kafrouni MI, et al. 2001. J. Immunol. 167:1566. Marguet D, et al. 1999. Immunity 11:231. Bashuda H, et al. 1997. Transplantation 63:113. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed Milstein O, et al. 2011. Blood 117:1042.
Product Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Azuma T, et al. 2008. Blood. 111:3635. PubMed Frey BF, et al. 2018. J Immunol. 200:1853. PubMed Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
RRID: AB_313583 (BioLegend Cat. No. 114502)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Ld/H-2Db haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 1496414972
UniProt: View information about H-2Ld/H-2Db on UniProt.org
Clone: 28-14-8
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (C3H/HeJ) IgG2a, κ