Biolegend, 114611, Alexa Fluor® 647 anti-mouse H-2K, 25μg

The 28-8-6 antibody reacts with the H-2K b and H-2D b MHC class I alloantigens expressed on nucleated cells from mice of the H-2K b /H-2D b haplotype. H-2K b /H-2D b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D d MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt) Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC) Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations: Capietto AH, et al. 2020. J Exp Med. 217:00:00. PubMed Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed Santharam MA, et al. 2023. Int J Mol Sci. 24:. PubMed Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed Li J, et al. 2020. Cancer Discov. . PubMed Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed Bui J, et al. 2006. J Immunol. 176:905. PubMed
RRID: AB_492931 (BioLegend Cat. No. 114612)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb/H-2Db haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.
Gene ID: 1496414972
UniProt: View information about H-2Kb/H-2Db on UniProt.org
Clone: 28-8-6
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (C3H) IgG2a, κ