Biolegend, 114712, Alexa Fluor® 647 anti-mouse H-2K, 100μg

The 34-1-2S antibody reacts with the H-2K d /H-2D d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K d /H-2D d haplotype. H-2K d /H-2D d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations: Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed Wu J, et al. 2009. Clin Cancer Res. 1.063888889. PubMed Dixit S, et al. 2018. Biomaterials. 159:130. PubMed Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
RRID: AB_493063 (BioLegend Cat. No. 114712)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kd/H-2Dd haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 1496414972
UniProt: View information about H-2Kd/H-2Dd on UniProt.org
Clone: 34-1-2S
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ