Biolegend, 114716, PerCP/Cyanine5.5 anti-mouse H-2K, 100μg

The 34-1-2S antibody reacts with the H-2K d /H-2D d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K d /H-2D d haplotype. H-2K d /H-2D d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations: Li X, et al. 2022. Nat Commun. 13:2794. PubMed
RRID: AB_2734175 (BioLegend Cat. No. 114715) AB_2734176 (BioLegend Cat. No. 114716)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kd/H-2Dd haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 1496414972
UniProt: View information about H-2Kd/H-2Dd on UniProt.org
Clone: 34-1-2S
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.