Biolegend, 114912, Alexa Fluor® 647 anti-mouse H-2K, 100μg

The 36-7-5 antibody reacts with the H-2K k MHC class I alloantigen expressed on nucleated cells from mice of the H-2K k haplotype. H-2K k is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A.AL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114914). Does not react with alloantigens of other haplotypes (e.g., b, d, q).
Application References(PubMed link indicates BioLegend citation): Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800. Johnson RM. 2004. Infect. Immun. 72:3951. Ardehali A, et al. 1995. Circulation 92:450.
Product Citations: Nakanishi Y, et al. 2018. Immunity. 49:1132. PubMed
RRID: AB_493115 (BioLegend Cat. No. 114912)
Structure: MHC class I
Distribution: Nucleated cells of the H-2kk haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972
UniProt: View information about H-2Kk on UniProt.org
Clone: 36-7-5
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (A.TL) IgG2a, κ