Product Description
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed Xu H, et al. 2023. Elife. 12:. PubMed Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed Song R, et al. 2022. Immunology. 165:274. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Friedman D, et al. 2022. J Immunol. 208:1845. PubMed Davis FM, et al. 2020. J Immunol. 2503:204. PubMed Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed Sasse C, et al. 2022. Pathogens. 11:. PubMed Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed She L, et al. 2021. JCI Insight. 6:e143509. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed Yu VWC, et al. 2017. Cell. 168:944. PubMed Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Hutter K, et al. 2020. FEBS J. . PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed Laubreton D, et al. 2020. Viruses. 12:00. PubMed Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_313638 (BioLegend Cat. No. 115503) AB_313639 (BioLegend Cat. No. 115504)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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