Biolegend, 115541, Brilliant Violet 650™ anti-mouse CD19 Antibody, 125microl

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Skladanowska K, et al. 2022. Cell Rep. 41:111490. PubMed Brunner SM, et al. 2023. Int J Mol Sci. 24:. PubMed Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed Kim EH, et al. 2020. Elife. 9:00. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Wei SC, et al. 2019. Immunity. 50:1084. PubMed Rojas OL et al. 2018. Cell. 176(3):610-624 . PubMed Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed Al Nabhani Z, et al. 2019. Immunity. 50:1276. PubMed Zhuo M, et al. 2021. Pigment Cell Melanoma Res. 34:605. PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Wei SC, et al. 2020. Cancer Discov. . PubMed Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed McKee AM, et al. 2021. iScience. 24:103012. PubMed Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed Deng M, et al. 2020. Nat Commun. 11:2193. PubMed Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed Muri J, et al. 2020. eLife. 9:e53627.. PubMed Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
RRID: AB_11204087 (BioLegend Cat. No. 115541)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ