Biolegend, 115606, PE anti-mouse CD314 (NKG2D) Antibody, 200μg

NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8 + T cells, γ/δ T cells and NK1.1 + T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The A10 antibody has been reported to costimulate NK cells.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Soluble mouse NKG2D
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: blocking and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115610).
Application References(PubMed link indicates BioLegend citation): Gilfillan S, et al. 2002. Nat Immunol. 3:1150. Ho EL, et al. 2002. J. Immunol. 169:3667.
Product Citations: Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
RRID: AB_313658 (BioLegend Cat. No. 115605) AB_313659 (BioLegend Cat. No. 115606)
Structure: C-type lectin
Distribution: NK, NK T
Function: NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
Receptors: Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Cell Type: NK cells, NKT cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Vance RE, et al. 1998. J. Exp. Med. 188:1841. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667.
Gene ID: 27007
UniProt: View information about CD314 on UniProt.org
Clone: A10
Regulatory Status: RUO
Other Names: CD314
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.