Biolegend, 115711, FITC anti-mouse CD314 (NKG2D) Antibody, 50μg

NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8 + T cells, γ/δ T cells and NK1.1 + T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Soluble mouse NKG2D
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported (for the relevant formats) applications include: blocking1,2,4,5 and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).
Application References(PubMed link indicates BioLegend citation): Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block) Ho EL, et al. 2002. J. Immunol. 169:3667. (Block) Maeda M, et al. 2004. J. Immunol. 172:6115. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block) O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block) Tang X, et al. 2006. J. Immunol. 177:7645. (FC)
Product Citations: Lin D, et al. 2016. Cancer Res . 76: 3179 - 3188. PubMed Song Y, et al. 2020. Front Immunol. 11:558143. PubMed Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed Kamizono S, et al. 2009. J Exp Med. 206:2977. PubMed
RRID: AB_2133291 (BioLegend Cat. No. 115711)
Structure: C-type lectin
Distribution: NK and NK-T cells
Function: NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
Ligand/Receptor: Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Cell Type: NK cells, NKT cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Vance RE, et al. 1998. J. Exp. Med. 188:1841. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667.
Gene ID: 27007
UniProt: View information about CD314 on UniProt.org
Clone: C7
Regulatory Status: RUO
Other Names: CD314
Isotype: Armenian Hamster IgG