Biolegend, 115812, APC anti-mouse CD126 (IL-6Rα chain) Antibody, 100μg

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: OKT4 hybridoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5, in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined to not react with rat.
Application References(PubMed link indicates BioLegend citation): Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP) Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP) Sun R, et al. 2004. J. Immunol. 172:5648. (FC) McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block) Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP) Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block) Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
Product Citations: Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed Figueiredo ML, et al. 2020. Mol Ther Methods Clin Dev. 17:739. PubMed Lee CG, et al. 2019. JCI Insight. 4:5. PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
RRID: AB_2127937 (BioLegend Cat. No. 115811) AB_2296238 (BioLegend Cat. No. 115812)
Structure: Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution: Activated T cells and B cells, monocytes, hepatocytes, plasma cells
Function: Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor: IL-6
Cell Type: B cells, Mesenchymal Stem Cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797. 2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London. 3. Boulanger MJ, et al. 2003. Science 300:2101.
Gene ID: 16194
UniProt: View information about CD126 on UniProt.org
Clone: D7715A7
Regulatory Status: RUO
Other Names: IL-6 Receptor α chain, IL-6R
Isotype: Rat IgG2b, κ