Biolegend, 116102, Purified anti-mouse CD54 Antibody, 500μg

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse NS-1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).
Application References(PubMed link indicates BioLegend citation): Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations: Yun S, et al. 2016. Nat Cell Biol. 18: 1043-1053. PubMed Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed Yu L, et al. 2022. Cancer Sci. 113:3980. PubMed Jeong HW, et al. 2022. Elife. 11:. PubMed Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Greene C, et al. 2022. Nat Commun. 13:2003. PubMed Reglero-Real N, et al. 2021. Immunity. :. PubMed Budatha M, et al. 2021. J Am Heart Assoc. 10:e021160. PubMed Greineder C, et al. 2013. PLoS One. 14:80110. PubMed Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed Mentrup T, et al. 2019. J Exp Med. 216:807. PubMed Lee JH, et al. 2022. iScience. 25:104166. PubMed Wolf D, et al. 2018. Nat Commun. 9:525. PubMed Yun S, et al. 2019. J Clin Invest. 130:4863. PubMed Douglas G, et al. 2019. Cardiovasc Res. :. PubMed Liu J, et al. 2010. Proc Natl Acad Sci U S A. 107:16530. PubMed El-Assaad F, et al. 2013. Infect Immun . 81:3984. PubMed Lanitis E, et al. 2021. J Immunother Cancer. 9:. PubMed Wu C, et al. 2020. J Immunol. 204:212. PubMed Matsui Y 2007. Radiology . 243:386. PubMed Echeverria GV, et al. 2018. Nat Commun. 9:5079. PubMed Mehta V, et al. 2021. Sci Adv. 7:. PubMed Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed Clark VD, et al. 2018. Brain Res. 1699:158. PubMed Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed Chacko A, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PubMed Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed Seidler D, et al. 2011. J Immunol. 187:6108. PubMed Chen PY, et al. 2019. Nat Metab. 0.675. PubMed Saitoh S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01687-x. PubMed Tran NL, et al. 2022. Elife. 11:. PubMed
RRID: AB_313692 (BioLegend Cat. No. 116101) AB_313693 (BioLegend Cat. No. 116102)
Structure: Ig superfamily, 90 kD
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function: Immune reaction, inflammation, adhesion
Ligand/Receptor: CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
Gene ID: 15894
UniProt: View information about CD54 on UniProt.org
Clone: YN1/1.7.4
Regulatory Status: RUO
Other Names: ICAM-1, Ly-47
Isotype: Rat IgG2b, κ