Biolegend, 116123, PerCP/Cyanine5.5 anti-mouse CD54 Antibody, 25μg

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse NS-1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).
Application References(PubMed link indicates BioLegend citation): Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations: Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
RRID: AB_2715951 (BioLegend Cat. No. 116123) AB_2715952 (BioLegend Cat. No. 116124)
Structure: Ig superfamily, 90 kD
Distribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
Function: Immune reaction, inflammation, adhesion
Ligand/Receptor: CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
Gene ID: 15894
UniProt: View information about CD54 on UniProt.org
Clone: YN1/1.7.4
Regulatory Status: RUO
Other Names: ICAM-1, Ly-47
Isotype: Rat IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.