Biolegend, 116303, FITC anti-mouse H-2K, 50μg

The AF3-12.1 antibody reacts with the H-2K k MHC class I alloantigen expressed on nucleated cells from mice of the H-2K k haplotype. H-2K k is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF3-12.1 antibody does not cross-react with other haplotypes (b, d, f, j, p, q, r, s, u, v).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A/J mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Does not react with other haplotypes (e.g. b, d, f, j, p, q, r, s, u, v).
Application References(PubMed link indicates BioLegend citation): Wasowska B, et al. 2001. Transplantation 71:727. Yamamoto M, et al. 1998. J. Immunol. 160:2188.
RRID: AB_313719 (BioLegend Cat. No. 116304)
Structure: MHC class I
Distribution: Nucleated cells of the H-2kk haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972
UniProt: View information about H-2Kk on UniProt.org
Clone: AF3-12.1
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG1, κ