Biolegend, 116412, Alexa Fluor® 647 anti-mouse I-A, 100μg

The AF6-120.1 antibody reacts with the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 k and H-2 u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL/10J splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5). Does not react with other haplotypes (e.g., d, f, q, r, s).
Application References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) Nakagawa TY, et al. 1999. Immunity 10:207. (IP) Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
Product Citations: Chang Y, et al. 2008. Blood. 111:5054. PubMed Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed Niven J, et al. 2019. Cell Rep. 28:21. PubMed Lopes N, et al. 2022. Elife. 11:. PubMed
RRID: AB_493141 (BioLegend Cat. No. 116412)
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
Gene ID: 14961
UniProt: View information about I-Ab on UniProt.org
Clone: AF6-120.1
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (BALB/c) IgG2a, κ