Biolegend, 116507, PE anti-mouse H-2K, 50μg

The AF6-88.5 antibody reacts with H-2K b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K b haplotype. H-2K b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
Application References(PubMed link indicates BioLegend citation): Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations: Kongsomboonvech AK, et al. 2020. PLoS Pathog. 16:e1008327. PubMed D'Alise AM, et al. 2022. Sci Transl Med. 14:eabo7604. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed Lu SX, et al. 2021. Cell. . PubMed Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed Sarvi S et al. 2018. Cell chemical biology. 25(12):1456-1469 . PubMed Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed Nair A, et al. 2007. Brain Behav Immun. 21:791. PubMed Zheng Y, et al. 2022. Transl Res. :. PubMed Patil ND, et al. 2022. Front Immunol. 13:818015. PubMed
RRID: AB_313734 (BioLegend Cat. No. 116507) AB_313735 (BioLegend Cat. No. 116508)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972
UniProt: View information about H-2Kb on UniProt.org
Clone: AF6-88.5
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.