Biolegend, 116705, PE anti-mouse CD235a (Glycophorin A) Antibody, 25μg

CD235a, also known as Glycophorin A (Gypa) is a type I transmembrane sialoglycoprotein that is expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells. Glycophorin A has been proposed to be involved in translocation of SLC4A1 to the plasma membrane. Glycophorin A may also be involved in regulation of response to osmotic stress.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Glycophorin A-transfectant cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S21003A does not block the binging of clone Ter-119 to target cells. Clone S21003A could induce RBCs aggregation at high concentration. Clone S21003A reacts with mouse CD235a (Glycophorin A).
RRID: AB_3674980 (BioLegend Cat. No. 116705) AB_3674980 (BioLegend Cat. No. 116706)
Structure: CD235a is type I transmembrane sialoglycoprotein
Distribution: Expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells
Function: Regulation of response to osmotic stress. May be involved in translocation of SLC4A1 to the plasma membrane
Cell Type: Erythrocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Arimitsu N, et al. 2003. Genes Cells. 8:769-777-77. Devin RB, et al. 2016. Proc Natl Acad Sci USA. 113:5245-50.
Gene ID: 14934
UniProt: View information about CD235a on UniProt.org
Clone: S21003A
Regulatory Status: RUO
Other Names: GPA, CD235a, GYPA, Sialoglycoprotein alpha, MN sialoglycoprotein, PAS-2, Monoclonal antibody to mouse Glycophorin A (GYPA / CD235a)
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.