Biolegend, 116810, Pacific Blue™ anti-mouse Ly-49A Antibody, 100μg

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2D d and H-2D k , triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Con A activated splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.
Application References(PubMed link indicates BioLegend citation): Takei F. 1983. J. Immunol. 130:2794. Chan P, et al. 1989. J. Immunol. 142:1727.
Product Citations: Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
RRID: AB_572013 (BioLegend Cat. No. 116810)
Structure: C-type lectin, Ly-49, ITIM, 44 kD
Distribution: NK and NK-T cells
Function: Inhibitory NK cell receptor
Ligand/Receptor: MHC Class I Molecules
Cell Type: NK cells, NKT cells
Biology Area: Immunology
Antigen References: 1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236. 2. Takei FJ, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67.
Gene ID: 16627
UniProt: View information about Ly-49A on UniProt.org
Clone: YE1/48.10.6
Regulatory Status: RUO
Other Names: Klra1, A1, killer cell lectin-like receptor, subfamily A, member 1
Isotype: Rat IgG2a, κ