Biolegend, 118803, PE/Cyanine7 anti-mouse CD319 Antibody, 25μg

CD319/CS1/CRACC/SLAMF7 (Signaling lymphocytic activation molecule family member7) is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily. It is expressed on most and low or absent on monocytes and basophils. CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules. CD319 is a self-ligand and homophilic interaction of CD319 regulates NK cell cytolytic activity. This mouse CD319 antibody does not show cross-reactivity with rat or human.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2941411 (BioLegend Cat. No. 118803) AB_2941411 (BioLegend Cat. No. 118804)
Structure: Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
Distribution: Spleen, lymph node, bone marrow, testis, thymus, NK cells, B cells, and subsets of T cells
Function: Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT)
Interaction: Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT)
Cell Type: B cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Li Y, et al. 2013. PLoS One. 8(9):e76681. Dongre P, et al. 2013. Mol Immunol. 54:254-63. Tovar V, et al. 2002. Immunogenetics. 54:394-402. Dong Z, et al. 2009. Nat Immunol. 10:973-80.
Gene ID: 75345
UniProt: View information about CD319 on UniProt.org
Clone: W19300I
Regulatory Status: RUO
Other Names: SLAMF7, CS1, CRACC, CD319, 19A
Isotype: Rat IgG2a, κ