Biolegend, 119105, PE anti-mouse CD255 (TWEAK) Antibody, 25μg

CD255, also known as TWEAK (TNF-related weak inducer of apoptosis), is a 27 kD protein and a tumor necrosis factor superfamily member 12 (TNFSF12). TWEAK is a type II transmembrane protein expressed on a variety of cell types. A soluble form of TWEAK is produced by proteolytic processing. TWEAK binds to the TWEAK receptor, FN14, to induce apoptosis, NF-κB translocation, and promote angiogenesis and endothelial cell proliferation. It has also been implicated in the induction of inflammatory cytokines.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant mouse CD255 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3106016 (BioLegend Cat. No. 119105) AB_3106016 (BioLegend Cat. No. 119106)
Structure: TNF superfamily member; type II membrane protein and secreted; homotrimer, 27 kD
Distribution: Widely expressed; induced on activated CD4+ cells
Function: Weak inducer of apoptosis in some cell types, activates NF-κB, promotes angiogenesis and endothelial cell proliferation, induces inflammatory cytokine production
Ligand/Receptor: TWEAKR/FN14
Cell Type: T cells
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Chicheportiche Y, et al. 1997. J. Biol. Chem. 272:32401-10. Schneider P, et al. 1999. Eur. J. Immunol. 29:1785-92.
Gene ID: 21944
UniProt: View information about CD255 on UniProt.org
Clone: A19079J
Regulatory Status: RUO
Other Names: TNFSF12, TNF-related weak inducer of apoptosis
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.