Biolegend, 120120, Brilliant Violet 421™ anti-mouse CD197 (CCR7) Antibody, 500microl

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.
500microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR7 transfected RBL-2H3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
Application References(PubMed link indicates BioLegend citation): Ohl L, et al. 2004. Immunity 21:279. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC) Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC) Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. Mao A et al. 2005. J. Immunol. 175:5146. PubMed Allende ML, et al. 2008. FASEB J. 22:307. PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed Chen H, et al. 2005. J. Immunol. 175:591. PubMed Florido M, et al. 2009. Immunobiology. 214:643. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations: Kedmi R, et al. 2022. Nature. 610:737. PubMed Chen H, et al. 2005. J Immunol. 175:591. PubMed Toomer G, et al. 2022. Viruses. 14:. PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Mariani SA, et al. 2019. Immunity. 50:1439. PubMed Hwang I, Kehrl C 2004. J Immunol. 179:439. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID: AB_10897811 (BioLegend Cat. No. 120119) AB_2561446 (BioLegend Cat. No. 120120)
Structure: Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution: Hematopoietic stem cells, T subsets, mature dendritic cells
Function: Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor: MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type: Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Schweickart VL, et al. 1994. Genomics 23:643. 2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118. 3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053. 4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
Gene ID: 12775
UniProt: View information about CD197 on UniProt.org
Clone: 4B12
Regulatory Status: RUO
Other Names: C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.