Biolegend, 120121, PE/Dazzle™ 594 anti-mouse CD197 (CCR7) Antibody, 25μg

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR7 transfected RBL-2H3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
Application References(PubMed link indicates BioLegend citation): Ohl L, et al. 2004. Immunity 21:279. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC) Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC) Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. Mao A et al. 2005. J. Immunol. 175:5146. PubMed Allende ML, et al. 2008. FASEB J. 22:307. PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed Chen H, et al. 2005. J. Immunol. 175:591. PubMed Florido M, et al. 2009. Immunobiology. 214:643. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations: Li J, et al. 2021. Front Immunol. 12:649285. PubMed Lu X, et al. 2020. Sci Transl Med. 12:. PubMed Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
RRID: AB_2564316 (BioLegend Cat. No. 120121) AB_2564317 (BioLegend Cat. No. 120122)
Structure: Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution: Hematopoietic stem cells, T subsets, mature dendritic cells
Function: Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor: MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type: Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Schweickart VL, et al. 1994. Genomics 23:643. 2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118. 3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053. 4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
Gene ID: 12775
UniProt: View information about CD197 on UniProt.org
Clone: 4B12
Regulatory Status: RUO
Other Names: C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype: Rat IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.