Biolegend, 120607, APC anti-mouse LPAM-1 (Integrin α4β7) Antibody, 25μg

DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4ß7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo.
Application References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. (Block) Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP) Bogetto L, et al. 2000. Blood 95:2397. (IHC)
Product Citations: Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed Kawano H, et al. 2016. Int Immunol. 28: 489 - 501. PubMed Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Ghaedi M, et al. 2020. The Journal of Experimental Medicine. 217(3):jem. PubMed Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
RRID: AB_10719833 (BioLegend Cat. No. 120607) AB_10730607 (BioLegend Cat. No. 120608)
Structure: Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Distribution: Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Function: Lymphocyte adhesion
Ligand/Receptor: VCAM-1 (CD106), MAdCAM-1, fibronectin
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Berlin C, et al. 1994. Cell 74:185. 3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243. 4. Hamann A, et al. 1994. J. Immunol. 152:3282.
Gene ID: 31114425713
UniProt: View information about LPAM-1 on UniProt.org
Clone: DATK32
Regulatory Status: RUO
Other Names: α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
Isotype: Rat IgG2a, κ