Biolegend, 120613, Brilliant Violet 421™ anti-mouse LPAM-1 (Integrin α4β7) Antibody, 50μg

DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4ß7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo.
Application References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. (Block) Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP) Bogetto L, et al. 2000. Blood 95:2397. (IHC)
RRID: AB_3683148 (BioLegend Cat. No. 120613)
Structure: Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Distribution: Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Function: Lymphocyte adhesion
Ligand/Receptor: VCAM-1 (CD106), MAdCAM-1, fibronectin
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Berlin C, et al. 1994. Cell 74:185. 3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243. 4. Hamann A, et al. 1994. J. Immunol. 152:3282.
Gene ID: 31114425713
UniProt: View information about LPAM-1 on UniProt.org
Clone: DATK32
Regulatory Status: RUO
Other Names: α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.