Biolegend, 121010, FITC anti-human/mouse integrin β7 Antibody, 100μg

Integrin β7 is a 130 kD glycoprotein, also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on majority of peripheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,α IEL /β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its lignd E-Cadherin. The FIB27 antibody has been reported to react with mouse and human β7 integrin and to block β7 integrin-mediated cell adhesion in in vitro and in vivo studies.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. (Block) Tidswell M, et al. 1997. J. Immunol. 159:1497. (Block)
Product Citations: Buffone A, et al. 2018. J Cell Sci. 131:. PubMed
RRID: AB_2129310 (BioLegend Cat. No. 121010)
Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution: CD49d/ß7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/ß7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
Function: Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor: CD49d/ß7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/ß7: E-cadherin
Cell Type: Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nature 372:190.
Gene ID: 164213695
UniProt: View information about Integrin beta7 on UniProt.org
Clone: FIB27
Regulatory Status: RUO
Other Names: β7 Integrin, integrin βp, ITGB7
Isotype: Rat IgG2a, κ