Biolegend, 121223, PerCP/Cyanine5.5 anti-mouse CD43 Activation-Associated Glycoform Antibody, 25μg

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 + B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse WEHI 274.3 myeloid tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB) Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB) Onami TM, et al. 2002. J. Immunol. 168:6022. van der Most RG, et al. 2003. Intl. Immunol. 15:119. Chu VT, et al. 2007. J. Immunol. 179:5947. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations: Wheeler B, et al. 2023. Elife. :12. PubMed Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed Halford W, et al. 2015. PLoS One. 10: 0145228. PubMed Xu AQ, et al. 2020. Elife. 9:00. PubMed Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
RRID: AB_893336 (BioLegend Cat. No. 121223) AB_2286556 (BioLegend Cat. No. 121224)
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor: CD54, Siglec-1
Cell Type: B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. van den Berg TK, et al. 2001. J. Immunol. 166:3637. 2. Moore T, et al. 1994. J. Immunol. 153:4978. 3. Onami TM, et al. 2002. J. Immunol. 168:6022. 4. Tong J, et al. 2004. J. Exp. Med. 199:1277. 5. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 20737
UniProt: View information about CD43 on UniProt.org
Clone: 1B11
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.