Biolegend, 121442, Alexa Fluor® 700 anti-mouse CD103 Antibody, 100μg

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin α IEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β 7 , CD103 is expressed as αE/β 7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse intestinal intraepithelial lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.
Application References(PubMed link indicates BioLegend citation): LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP) Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF) Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations: Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID: AB_2813992 (BioLegend Cat. No. 121441) AB_2813993 (BioLegend Cat. No. 121442)
Structure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor: E-Cadherin
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
Gene ID: 16407
UniProt: View information about CD103 on UniProt.org
Clone: 2E7
Regulatory Status: RUO
Other Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype: Armenian Hamster IgG