Biolegend, 121625, PerCP/Cyanine5.5 anti-mouse CD107a (LAMP-1) Antibody, 25μg

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed Park S, et al. 2021. Front Immunol. 11:620166. PubMed Kim C, et al. 2019. Cell Rep. 29:2202. PubMed van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed Lind L, et al. 2021. J Neurovirol. 27:145. PubMed López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed Li Y, et al. 2022. Theranostics. 12:5364. PubMed Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed Zhang X, et al. 2019. Front Immunol. 10:744. PubMed
RRID: AB_2572054 (BioLegend Cat. No. 121625) AB_2572055 (BioLegend Cat. No. 121626)
Structure: LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID: 16783
UniProt: View information about CD107a on UniProt.org
Clone: 1D4B
Regulatory Status: RUO
Other Names: Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.