Biolegend, 122501, Purified anti-mouse Ly-6A/E (Sca-1) Antibody, 50μg

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mouse pre-T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F, IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
Application References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
Product Citations: Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed Lee JH, et al. 2022. iScience. 25:104166. PubMed Gronowicz G, et al. 2017. Tissue Eng Part A. 23:1490. PubMed Yao L, et al. 2021. J Bone Miner Res. 36:1159. PubMed Felthaus O, et al. 2010. Neurosci Lett. 471:179. PubMed Brandl C, et al. 2009. Exp Eye Res. 89:209. PubMed Martin K,et al. 2017. J Immunol.. 10.4049/jimmunol.1601522. PubMed Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed Shah K, et al. 2022. Nat Commun. 13:1827. PubMed Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed Ishii H, et al. 2018. Nat Commun. 2.188194444. PubMed Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed Zhang C, et al. 2018. Cell Res. 28:323. PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID: AB_756186 (BioLegend Cat. No. 122501) AB_756187 (BioLegend Cat. No. 122502)
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454
UniProt: View information about Ly-6A/E on UniProt.org
Clone: E13-161.7
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG2a, κ