Biolegend, 122523, PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) Antibody, 25μg

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mouse pre-T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
Application References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
Product Citations: Mo C, et al. 2022. EMBO J. 41:e108415. PubMed Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Clapes T, et al. 2021. Nat Cell Biol. 23:704. PubMed Adapala NS, et al. 2020. eLife. 9:e56095.. PubMed Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed Shamsi F, et al. 2020. Nat Commun. 11:1421. PubMed Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
RRID: AB_893621 (BioLegend Cat. No. 122523) AB_893617 (BioLegend Cat. No. 122524)
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454
UniProt: View information about Ly-6A/E on UniProt.org
Clone: E13-161.7
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.