Biolegend, 123124, Pacific Blue™ anti-mouse F4/80 Antibody, 100μg

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
Application References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Bucher K, et al. 2015. J Leukoc Biol. 2015;98: 365-372. PubMed Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed Gerlach BD, et al. 2021. Cell Metab. 33:2445. PubMed Xin B, et al. 2022. Hepatology. 76:630. PubMed Zhou Y, et al. 2022. Br J Pharmacol. 179:1978. PubMed Du Y, et al. 2022. FASEB J. 36:e22248. PubMed Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed Schneider C, et al. 2018. Cell. 174:271. PubMed Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed Tanaka Y, et al. 2015. PLoS One. 10: 0138621. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed Marangoni F, et al. 2021. Cell. . PubMed Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed Ni P, et al. 2014. J Immunol . 193:1778. PubMed Billi AC, et al. 2019. JCI Insight. 4:8. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Dichtl S, et al. 2021. Sci Adv. 7: . PubMed Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed Goncalves R, et al. 2011. J Exp Med. 208:1253. PubMed Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Haase C, et al. 2022. Nat Methods. 19:1622. PubMed Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed Okamura T, et al. 2021. Front Immunol. 12:669629. PubMed Dar HH, et al. 2022. JCI Insight. 7:. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Cohen H, et al. 2015. J Immunol. 195: 3828 - 3837. PubMed Stolt C, et al. 2016. J Immunol. 197: 834 - 846. PubMed Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed Boukelmoune N, et al. 2021. Brain Behav Immun. 93:43. PubMed Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed Jones M, et al. 2010. J Immunol. 185:3583. PubMed O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed Flores AM, et al. 2020. Nat Nanotechnol. 0.731944444. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed Serr I, et al. 2016. Nat Commun. 7:10991. PubMed Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed Fotakis P, et al. 2019. Arterioscler Thromb Vasc Biol. 39:e253. PubMed
RRID: AB_893487 (BioLegend Cat. No. 123123) AB_893475 (BioLegend Cat. No. 123124)
Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733
UniProt: View information about F4/80 on UniProt.org
Clone: BM8
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ