Biolegend, 123140, Alexa Fluor® 594 anti-mouse F4/80 Antibody, 100μg

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 5.0 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
Application References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed Ammassam Veettil R, et al. 2021. Pharmaceutics. 13:. PubMed Grandjean CL, et al. 2021. Sci Adv. 7:. PubMed Liang Y, et al. 2022. Dev Cell. 57:398. PubMed Halin C, et al. 2022. EMBO J. 41:e111528. PubMed Kim JK, et al. 2022. Cell Rep Methods. 2:100353. PubMed Gershater M, et al. 2022. J Immunol. 208:1595. PubMed Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed Xu DQ, et al. 2021. Acta Pharmacol Sin. 42:1080. PubMed Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed Bonnardel J, et al. 2019. Immunity. 51:638. PubMed Peauroi EM, et al. 2022. iScience. 25:105510. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Du J, et al. 2021. Mol Med Rep. 23:. PubMed Fu Y, et al. 2017. Biochem Pharmacol. 10.1016/j.bcp.2017.03.013. PubMed Tu W, et al. 2021. Cell Death Dis. 12:882. PubMed Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed Fan ZP, et al. 2021. Front Immunol. 12:743354. PubMed Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
RRID: AB_2563241 (BioLegend Cat. No. 123140)
Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733
UniProt: View information about F4/80 on UniProt.org
Clone: BM8
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ