Biolegend, 123149, Brilliant Violet 650™ anti-mouse F4/80 Antibody, 50μg

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
Application References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Wang C, et al. 2021. Cell Stem Cell. . PubMed Francian A, et al. 2021. J Drug Target. 29:754. PubMed Law HL, et al. 2020. Front Pharmacol. 11:274. PubMed Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed Rhys HI, et al. 2018. EBioMedicine. 29:60. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed Tang S, et al. 2022. Virol J. 19:32. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Zhang L, et al. 2020. Cell. 442:181. PubMed Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed Chen KE, et al. 2021. J Immunol. 206:141. PubMed Chen KE, et al. 2021. J Neuroinflammation. 18:140. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_2564589 (BioLegend Cat. No. 123149)
Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733
UniProt: View information about F4/80 on UniProt.org
Clone: BM8
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ