Biolegend, 123407, FITC anti-mouse CD21/CD35 (CR2/CR1) Antibody, 50μg

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Xu J, et al. 2021. Nature. 595:278. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed Xu M, et al. 2013. J Immunol. 190:5436. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Chen RJ, et al. 2022. iScience. 25:105595. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Chauvet S, et al. 2016. J Am Soc Nephrol. 27: 1665 - 1677. PubMed
RRID: AB_940403 (BioLegend Cat. No. 123407) AB_940405 (BioLegend Cat. No. 123408)
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand/Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
Gene ID: 1290212946
UniProt: View information about CD21/CD35 on UniProt.org
Clone: 7E9
Regulatory Status: RUO
Other Names: CR2/CR1
Isotype: Rat IgG2a, κ