Biolegend, 123409, PE anti-mouse CD21/CD35 (CR2/CR1) Antibody, 50μg

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed Prokopec K, et al. 2016. J Immunol. 197: 2063 - 2068. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed Liu R, et al. 2018. Front Immunol. 9:592. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Liu R, et al. 2021. Mol Cell Biol. 41:e0025121. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
RRID: AB_940411 (BioLegend Cat. No. 123409) AB_940413 (BioLegend Cat. No. 123410)
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand/Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
Gene ID: 1290212946
UniProt: View information about CD21/CD35 on UniProt.org
Clone: 7E9
Regulatory Status: RUO
Other Names: CR2/CR1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.