Biolegend, 123423, Alexa Fluor® 647 anti-mouse CD21/CD35 (CR2/CR1) Antibody, 25μg

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Trindade BC, et al. 2021. Immunity. 54:2273. PubMed Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
RRID: AB_2629577 (BioLegend Cat. No. 123423) AB_2629578 (BioLegend Cat. No. 123424)
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand/Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
Gene ID: 1290212946
UniProt: View information about CD21/CD35 on UniProt.org
Clone: 7E9
Regulatory Status: RUO
Other Names: CR2/CR1
Isotype: Rat IgG2a, κ