Biolegend, 123608, Alexa Fluor® 647 anti-mouse CD104 Antibody, 100μg

CD104 is a 205 kD type I transmembrane glycoprotein, also known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) to form the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells, and a subset of pre-T cells. The CD49f/CD104 heterodimer is an adhesion receptor for laminins (especially laminin 5) and keratin filaments, and is involved in the regulation of hemidesmosome formation and cell proliferation and activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Tumor-associated antigen TSP-180 from BALB/c lung carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemical staining1.
Application References(PubMed link indicates BioLegend citation): Tennenbaum T, et al. 1993. Cancer Res. 53:4803. (IHC)
Product Citations: Weiner AI 2022. Cell Reports. 41(11):111805. PubMed Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed Guo L, et al. 2019. Mol Cell. 73:815. PubMed Hara T, et al. 2022. Cell Mol Gastroenterol Hepatol. 13:1449. PubMed
RRID: AB_2563511 (BioLegend Cat. No. 123607) AB_2563512 (BioLegend Cat. No. 123608)
Structure: Integrin β chain, 205 kD, forms heterodimer with integrin α6 (CD49f)
Distribution: Epithelial cells, endothelial cells, some tumor cells, pre-T cell subset
Function: Adhesion
Ligand/Receptor: Laminins, keratin filaments
Cell Type: Embryonic Stem Cells, Endothelial cells, Epithelial cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kennel SJ, et al. 1992. J. Cell Sciences 101:1992.
Gene ID: 192897
UniProt: View information about CD104 on UniProt.org
Clone: 346-11A
Regulatory Status: RUO
Other Names: Integrin β4, β4 integrin, TSP-1180, ITGB4
Isotype: Rat IgG2a, κ