Biolegend, 124329, GoInVivo™ Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody, 25mg

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.
25mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation: The GoInVivo™ antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage. View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Maier H, et al. 2007. J. Immunol. 178:2714. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC) Paterson AM, et al. 2011. J. Immunol. 187:1097. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block) Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC) Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations: Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Bazan-Peregrino M, et al. 2021. J Immunother Cancer. 9:. PubMed Chen H, et al. 2022. J Immunother Cancer. 10:. PubMed Li D, et al. 2021. Mol Cancer Ther. 20:1926. PubMed Li H, et al. 2021. Nat Commun. 12:2773. PubMed Jaynes JM, et al. 2020. Sci Transl Med. 12:00. PubMed Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Wang C, et al. 2018. Sci Transl Med. 10. PubMed Yamazaki K, et al. 2022. Sci Rep. 12:3242. PubMed
RRID: AB_2566281 (BioLegend Cat. No. 124328) AB_2566282 (BioLegend Cat. No. 124329) AB_2566283 (BioLegend Cat. No. 124330) AB_2566092 (BioLegend Cat. No. 124325) AB_2566093 (BioLegend Cat. No. 124326) AB_2566094 (BioLegend Cat. No. 124327)
Structure: 40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution: T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Ligand/Receptor: PD-1 (PDCD1)
Cell Type: B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.
Gene ID: 60533
UniProt: View information about CD274 on UniProt.org
Clone: 10F.9G2
Regulatory Status: RUO
Other Names: B7-H1, PD-L1
Isotype: Rat IgG2b, κ