Biolegend, 124911, Alexa Fluor® 647 anti-mouse DC Marker (33D1) Antibody, 25μg

33D1 antigen, also known as DCIR2 (dendritic cell inhibitory receptor 2), is reported to be a dendritic cell marker on subpopulation DC in the spleen, thymus, and Peyer's patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4. Binding of clone 33D1 is calcium-dependent. We recommend staining in EDTA-free FACS buffer containing a source of Ca2+ ions.
Application References(PubMed link indicates BioLegend citation): Crowley MT, et al. 1990. J. Immunol. Methods 133:55. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF) Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC)
Product Citations: Wadley AJ, et al. 2018. J Appl Physiol (1985). 586:125. PubMed Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
RRID: AB_1227623 (BioLegend Cat. No. 124912)
Structure: The antigen recognized by 33D1 is yet unknown.
Distribution: 33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.
Function: GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.
Cell Type: Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: NA
UniProt: View information about 33D1 on UniProt.org
Clone: 33D1
Regulatory Status: RUO
Other Names: Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)
Isotype: Rat IgG2b, κ