Biolegend, 125011, PE/Cyanine7 anti-mouse FR4 (Folate Receptor 4) Antibody, 25μg

Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Yamaguchi T et al. 1007:Immunity 27(1)145
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
RRID: AB_1134199 (BioLegend Cat. No. 125012)
Structure: 28 kd protein consisting of 244 amino acids. Heavily glycosylated
Distribution: Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
Function: Mediate calcium-independent homophlic cell-cell adhesion
Ligand/Receptor: Folic acid and its derivative
Cell Type: Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Yamaguchi T et al 2007. Immunity 27:145 2. Spiegelstein O et al. 2000 Gene 258:117
Gene ID: 64931
UniProt: View information about FR4 on UniProt.org
Clone: 12A5
Regulatory Status: RUO
Other Names: Folate Receptor 4, FBP, FR δ
Isotype: Rat IgG1, κ