Biolegend, 125202, Purified anti-mouse CD223 (LAG-3) Antibody, 500μg

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine CD223-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed Wei SC, et al. 2019. Immunity. 50:1084. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID: AB_961187 (BioLegend Cat. No. 125202)
Structure: Ig superfamily
Distribution: T cells and NK cells, as well as T regulatory cells
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
Gene ID: 16768
UniProt: View information about CD223 on UniProt.org
Clone: C9B7W
Regulatory Status: RUO
Other Names: LAG3, LAG-3
Isotype: Rat IgG1, κ