Biolegend, 125212, PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3) Antibody, 100μg

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine CD223-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Nguyen T, et al. 2023. Nat Commun. 14:3357. PubMed Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed Balood M, et al. 2022. Nature. 611:405. PubMed Liu M, et al. 2022. Cells. 11: . PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Ertl Y 2014. J Immunol. 193:1836. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
RRID: AB_2561516 (BioLegend Cat. No. 125211) AB_2561517 (BioLegend Cat. No. 125212)
Structure: Ig superfamily
Distribution: T cells and NK cells, as well as T regulatory cells
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
Gene ID: 16768
UniProt: View information about CD223 on UniProt.org
Clone: C9B7W
Regulatory Status: RUO
Other Names: LAG3, LAG-3
Isotype: Rat IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.