Biolegend, 125225, PE/Cyanine7 anti-mouse CD223 (LAG-3) Antibody, 25μg

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine CD223-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations: Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed Wu M, et al. 2021. Nat Commun. 12:3500. PubMed Cribioli E, et al. 2022. Front Immunol. 13:976628. PubMed Pein M, et al. 2020. Nat Commun. 11:1494. PubMed He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed He X, et al. 2021. J Immunother Cancer. 9:. PubMed Molgora M, et al. 2020. Cell. 182:886. PubMed Truckenbrod EN, et al. 2021. Elife. 10:. PubMed Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
RRID: AB_2715763 (BioLegend Cat. No. 125225) AB_2715764 (BioLegend Cat. No. 125226)
Structure: Ig superfamily
Distribution: T cells and NK cells, as well as T regulatory cells
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
Gene ID: 16768
UniProt: View information about CD223 on UniProt.org
Clone: C9B7W
Regulatory Status: RUO
Other Names: LAG3, LAG-3
Isotype: Rat IgG1, κ