Biolegend, 125227, Brilliant Violet 650™ anti-mouse CD223 (LAG-3) Antibody, 50μg

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine CD223-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations: Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed Krishnamurty AT, et al. 2022. Nature. 611:148. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed Dammeijer F, et al. 2020. Cancer Cell. 38(5):685-700.e8. PubMed
RRID: AB_2687209 (BioLegend Cat. No. 125227)
Structure: Ig superfamily
Distribution: T cells and NK cells, as well as T regulatory cells
Cell Type: NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
Gene ID: 16768
UniProt: View information about CD223 on UniProt.org
Clone: C9B7W
Regulatory Status: RUO
Other Names: LAG3, LAG-3
Isotype: Rat IgG1, κ