Biolegend, 126310, PE anti-mouse CD357 (GITR) Antibody, 100μg

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25 + CD4 + Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25 + CD4 + Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD25+ CD4+ T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
Product Citations: Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Lacal P, et al. 2013. J Pharmacol Exp Ther. 347:164. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID: AB_1089132 (BioLegend Cat. No. 126309) AB_1089130 (BioLegend Cat. No. 126310)
Structure: 66-70 kD homodimer of TNFR superfamily member.
Distribution: Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
Function: Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor: GITR ligand (GITRL).
Cell Type: B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Shimizu J, et al. 2002 Nat. Immunol. 3:135. 3. Stephens GL, et al. 2004. J. Immunol. 173:5008. 4. McHugh RS, et al. 2002. Immunity 16:311.
Regulation: Activation of T and B cells can upregulate GITR expression.
Gene ID: 21936
UniProt: View information about CD357 on UniProt.org
Clone: DTA-1
Regulatory Status: RUO
Other Names: Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.