Biolegend, 126312, APC anti-mouse CD357 (GITR) Antibody, 100μg

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25 + CD4 + Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25 + CD4 + Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD25+ CD4+ T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
Product Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
RRID: AB_1134212 (BioLegend Cat. No. 126311) AB_2271858 (BioLegend Cat. No. 126312)
Structure: 66-70 kD homodimer of TNFR superfamily member.
Distribution: Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
Function: Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor: GITR ligand (GITRL).
Cell Type: B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Shimizu J, et al. 2002 Nat. Immunol. 3:135. 3. Stephens GL, et al. 2004. J. Immunol. 173:5008. 4. McHugh RS, et al. 2002. Immunity 16:311.
Regulation: Activation of T and B cells can upregulate GITR expression.
Gene ID: 21936
UniProt: View information about CD357 on UniProt.org
Clone: DTA-1
Regulatory Status: RUO
Other Names: Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype: Rat IgG2b, κ