Biolegend, 126317, PE/Cyanine7 anti-mouse CD357 (GITR) Antibody, 25μg

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25 + CD4 + Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25 + CD4 + Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD25+ CD4+ T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
Product Citations: Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
RRID: AB_2563385 (BioLegend Cat. No. 126317) AB_2563386 (BioLegend Cat. No. 126318)
Structure: 66-70 kD homodimer of TNFR superfamily member.
Distribution: Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
Function: Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor: GITR ligand (GITRL).
Cell Type: B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Shimizu J, et al. 2002 Nat. Immunol. 3:135. 3. Stephens GL, et al. 2004. J. Immunol. 173:5008. 4. McHugh RS, et al. 2002. Immunity 16:311.
Regulation: Activation of T and B cells can upregulate GITR expression.
Gene ID: 21936
UniProt: View information about CD357 on UniProt.org
Clone: DTA-1
Regulatory Status: RUO
Other Names: Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype: Rat IgG2b, κ