Biolegend, 126333, Brilliant Violet 711™ anti-mouse CD357 (GITR) Antibody, 50μg

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25 + CD4 + Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25 + CD4 + Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD25+ CD4+ T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
RRID: AB_3097506 (BioLegend Cat. No. 126333)
Structure: 66-70 kD homodimer of TNFR superfamily member.
Distribution: Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
Function: Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor: GITR ligand (GITRL).
Cell Type: B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Shimizu J, et al. 2002 Nat. Immunol. 3:135. 3. Stephens GL, et al. 2004. J. Immunol. 173:5008. 4. McHugh RS, et al. 2002. Immunity 16:311.
Regulation: Activation of T and B cells can upregulate GITR expression.
Gene ID: 21936
UniProt: View information about CD357 on UniProt.org
Clone: DTA-1
Regulatory Status: RUO
Other Names: Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype: Rat IgG2b, κ